1DDB.pdb (Mouse BID — example) uploaded. Select chains and fluorophore below.
Select which protein chains to analyse for labelling sites.
Scores residue pairs for optimal donor–acceptor placement.
Ranks every residue as a potential labelling site for a single fluorophore.
The fluorescent dye you plan to attach. Each has different physical dimensions that affect which residues are accessible.
Run a selection of common donor–acceptor FRET pairs and compare their top residue pairs side-by-side.
These are widely-used combinations, not an exhaustive set — the best pair depends on your Förster radius (R₀) and instrument. To score a different pair, just choose the donor and acceptor above and run a single analysis. Comparing all takes roughly 8× a single run; the individual dye selection is ignored when this box is checked.
Running Analysis…
This may take a minute or two.
