Configure — LabelWise

Configure Analysis

1OMP.pdb (MBP open — apo) uploaded. + 1ANF.pdb (MBP closed — holo) (holo). Select chains and fluorophore below.

Chains

Select which protein chains to analyse for labelling sites.

Chain A

Single-molecule FRET

Scores residue pairs for optimal donor–acceptor placement.

Label Site Scoring

Ranks every residue as a potential labelling site for a single fluorophore.

FRET Dye Pair

The fluorescent dye you plan to attach. Each has different physical dimensions that affect which residues are accessible.

Donor Dye

Acceptor Dye

Compare Dye Pairs

Run a selection of common donor–acceptor FRET pairs and compare their top residue pairs side-by-side.

Run 8 common FRET pairs (Cy3/Cy5, Atto550/Atto647N, Alexa546/Alexa647, …)

These are widely-used combinations, not an exhaustive set — the best pair depends on your Förster radius (R₀) and instrument. To score a different pair, just choose the donor and acceptor above and run a single analysis. Comparing all takes roughly 8× a single run; the individual dye selection is ignored when this box is checked.

Typically takes 10–60 seconds depending on protein size.